Experimental study on the effect of Hydroxycamptothecine-loaded Nanoparticles on tongue squamous cell carcinoma Tca8113 cell line

doi:10.3877 / cma.j.issn.1674-1366.2009-03-005

Abstract

Abstract:

Objective

To improve the pharmaceutical dosage form of Hydroxycamptothecine （HCPT） and to explore its suppressive effects and inhibitive mechanism on tongue squamous cell carcinoma Tca8113 cell line in vitro.

Methods

The closed ring form HCPT-loaded PEG-PBLG nanoparticles were synthesized and tested. After affected by HCPT and HCPT/PEGPBLG respectively in vitro， Tca8113 cells' morphologic changes were observed， cytotoxicity effects were evaluated by MTT assay， the cell cycle and apoptosis changes were assessed with flow cytometry （FCM） and DNA gel electrophoresis.

Results

HCPT/PEG-PBLG nanoparticles had been successfully synthesized， with 56.8% in encapsulation efficiency and 7.5% in loading level. Tca8113 cells suppression and apoptosis were observed both in HCPT and HCPT/PEGPBLG group under microscope. Results of MTT assay showed that HCPT had been obvious toxic to Tca8113 cells. HCPT/PEG-PBLG showed similar effectiveness but relatively delayed at check point to that of HCPT： GI value was less than that of HCPT at 48 hours （P≤0.01） and equal to HCPT at 96 hours （no statistical significance was found）（P＞0.05）. FCM detected cell cycle variation： in HCPT groups， cells had been retarded in S phase and apoptosis rate raised.Compared with HCPT， HCPT/PEG-PBLG had similar effects while the velocity of the effectiveness was slow. DNA extraction and gel electrophoresis demonstrated classical DNA ladder mark of apoptosis induced by HCPT and HCPT/PEG-PBLG.

Conclusion

HCPT appeared to fairly restrained tumor growth of Tca8113 cell in vitro： delayed cell cycle in S phase and induced apoptosis. HCPT/ PEG-PBLG nanoparticles slowed down the drug release. So， compared wih HCPT， it showed more slight reactions and achieved the same final anti-tumor effects.

Key words: Hydroxycamptothecine （HCPT）, Poly ［ethylene glycol］（PEG）, Poly［γbenzyl-L-glutamate］（PBLG）, Oral Squamous Cell Carcinoma（OSCC）, Nanoparticles

Dan CHEN, Li-ping CHAI, Ji WANG, Wangxiang YAN, An-xun WANG, Su LI, Yu CHEN, Xue-qiang DING. Experimental study on the effect of Hydroxycamptothecine-loaded Nanoparticles on tongue squamous cell carcinoma Tca8113 cell line[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2009, 3(03): 262-269.

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Figures/Tables 6

References 16

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浓度（mol/L）	空白对照组0	HCPT		HCPT/PEG-PBLG
浓度（mol/L）	空白对照组0	1×10^-8	1×10^-6	1×10^-8	1×10^-6
G0/G1期
24 h	60.60	58.90	7.00	55.70	66.40
48 h	59.10	13.60	0.00	64.70	2.70
72 h	45.30	2.10	0.00	18.80	0.60
S期
24 h	29.20	37.00	92.80	39.90	32.70
48 h	32.80	86.30	100.00	33.00	96.90
72 h	41.40	91.60	100.00	81.20	98.90
G2/M期	10.30	4.10	0.20	4.40	0.90
24 h	10.30	4.10	0.20	4.40	0.90
48 h	8.10	0.20	0.00	2.30	0.40
72 h	13.30	6.30	0.00	0.00	0.50
凋亡率
24 h	2.10	7.50	12.40	7.30	8.90
48 h	3.50	13.60	17.90	9.40	16.70
72 h	4.20	22.80	27.70	9.30	26.90

浓度（mol/L）	空白对照组0	HCPT		HCPT/PEG-PBLG
浓度（mol/L）	空白对照组0	1×10^-8	1×10^-6	1×10^-8	1×10^-6
G0/G1期
24 h	60.60	58.90	7.00	55.70	66.40
48 h	59.10	13.60	0.00	64.70	2.70
72 h	45.30	2.10	0.00	18.80	0.60
S期
24 h	29.20	37.00	92.80	39.90	32.70
48 h	32.80	86.30	100.00	33.00	96.90
72 h	41.40	91.60	100.00	81.20	98.90
G2/M期	10.30	4.10	0.20	4.40	0.90
24 h	10.30	4.10	0.20	4.40	0.90
48 h	8.10	0.20	0.00	2.30	0.40
72 h	13.30	6.30	0.00	0.00	0.50
凋亡率
24 h	2.10	7.50	12.40	7.30	8.90
48 h	3.50	13.60	17.90	9.40	16.70
72 h	4.20	22.80	27.70	9.30	26.90

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